CatalogueChromatographyGas chromatographyGC reagentsSilylation reagents - BSTFA, SILYL-991
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Silylation reagents - BSTFA, SILYL-991

For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
N,O-bis-trimethylsilyl-trifluoroacetamide
m.w. 257.4, Bp 40°C (12mm Hg), density d20°/4° = 0.961
BSTFA: R = CF3 powerful trimethylsilyl donor with approximately the same donor strength as the non-fluorinated analogue BSA
advantage of BSTFA over BSA: greater volatility of its reaction products (particularly useful for GC of some lower boiling TMS amino acids.

BSTFA is nonpolar (less polar than MSTFA), and can be mixed with acetonitrile for improved solubility. For silylating fatty acid amides, hindered hydroxyls and other compounds, which are difficult to silylate (like secondary alcohols and amines), we recommend BSTFA + 1% trimethylchlorosilane (TMCS), available under the designation SILYL-991.
Silylation reagents - BSTFA, SILYL-991


Silylation reagents - BSTFA, SILYL-991
Data
Bestell Nr.
GHS
Signalwort
H-Satz
P-Satz
UN/Nr.
Bestell Nr.
4.001 486
GHS
Signalwort
Warning
H-Satz
H226 | H315 | H319
P-Satz
P210 | P280sh
UN/Nr.
3316
Bestell Nr.
6.803 320
GHS
Signalwort
Warning
H-Satz
H226 | H315 | H319
P-Satz
P210 | P280sh
UN/Nr.
3316
Bestell Nr.
4.001 487
GHS
Signalwort
Warning
H-Satz
H226 | H315 | H319
P-Satz
P210 | P280sh
UN/Nr.
3316
Bestell Nr.
4.001 511
GHS
Signalwort
Warning
H-Satz
H226 | H315 | H319
P-Satz
P210 | P280sh
UN/Nr.
3316
Bestell Nr.
4.001 510
GHS
Signalwort
Warning
H-Satz
H226 | H315 | H319
P-Satz
P210 | P280sh
UN/Nr.
3316
Bestell Nr.
4.001 509
GHS
Signalwort
Warning
H-Satz
H226 | H315 | H319
P-Satz
P210 | P280sh
UN/Nr.
3316
 DescriptionCapacity
ml
PK 
 BSTFAProd.No.4.001 486120
 BSTFAProd.No.6.803 320101
 BSTFAProd.No.4.001 487105
 SILYL-991 (BSTFA - TMCS (99:1)Prod.No.4.001 511120
 SILYL-991 (BSTFA - TMCS (99:1)Prod.No.4.001 510501
 SILYL-991 (BSTFA - TMCS (99:1)Prod.No.4.001 5091001
Due to their purpose, derivatisation reagents are very reactive substances. For this reason they should be stored cool and protected from moisture.
The derivatisation reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability and should be used immediately.

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